mouse ngf β elisa kit  (Boster Bio)

Journal: CNS Neuroscience & Therapeutics

Article Title: hUC ‐ MSCs via β‐ NGF Alleviate Cognitive Impairment After Tibial Fracture Surgery by Regulating the STMN2 / NMNAT2 ‐ SARM1 ‐ NF ‐ κB Signaling Pathway

doi: 10.1002/cns.71062

Figure Lengend Snippet: hUC‐MSCs exert therapeutic effects in the PND mouse model via β‐NGF. (A) 20‐Human Neuro Discovery array analysis between blank‐medium and MSC‐conditioned medium (MSC‐CM) (B) ELISA quantification of β‐NGF levels in blank‐medium and MSC‐CM ( n = 3). (C, D) Representative Western blot bands and quantitative analysis of β‐NGF protein expression in hippocampal tissues from CON, CON‐MSC, SUR, and SUR‐MSC groups. (E) Total distance traveled by mice in OFT ( n = 9). (F) Alternative corrections (%) in Y‐maze test ( n = 9). (G) Novel object preference (%) in NOR ( n = 9). (H) Representative GFAP, NeuN and IBA1 immunostaining images of hippocampal CA1 region. (I) Representative Golgi staining images (J) Quantitative analysis of NeuN positive area percentage in hippocampal CA1 region ( n = 6). (K) Quantitative analysis of GFAP positive area percentage in hippocampal CA1 region ( n = 6). (L) Quantitative analysis of IBA1 positive area percentage in hippocampal CA1 region ( n = 6). (M) Quantitative analysis of dendritic spine density in hippocampal CA1 region. Representative Western blot bands (N) and quantitative analysis of β‐NGF (O), p‐TrkA/TrkA (P), STMN2 (Q) and NMNAT2 (R) protein expression in hippocampal tissues from CON, SUR, SUR‐MSC NC and SUR‐MSC NGFsiRNA groups ( n = 6). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The supernatant was harvested following the manufacturer's instructions, and the concentrations of IL‐1β (HUABIO, China, EM0001), IL‐6 (LIANKE, China, EK206), TNF‐α (HUABIO, China, EM0010), mouse (HUABIO, China, RK00154), and human β‐NGF (Abclonal, China, RK03064) were quantified using ELISA kits.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Immunostaining, Staining

Journal: Molecular neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood-Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats.

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: Fig. 2 The protein of mNGF expression in the M1 and various cognitive-related brain regions as measured by ELISA. A, B, C, D, E, F The NGF + SMES group had signifi- cant effect except PrL and MS. The results are demonstrated as the mean ± SEM (n = 5, each group).*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. NGF + SMES group. G The hippocampus had significant effect. The results are dem- onstrated as the mean ± SEM (n = 5, each group). **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. hippocampus group. a, b, c, d, e, f Compared with the MCAO/R group, NGF group, or NGF + SMES + PDTC group, NGF + SMES group had significant permeation effect in the M1, brain stem, and hippocampus. The results are demonstrated as the mean ± SEM (n = 5 or 8, each group). *P < 0.05, **P < 0.01, and ****P < 0.0001 vs. NGF + SMES group. g The hip- pocampus had significant effect. The results are demonstrated as the mean ± SEM (n = 6, each group). ****P < 0.0001 vs. hip- pocampus group

Article Snippet: Enzyme‐Linked Immunosorbent Assay Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse BetaNGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular Neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood–Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: Experimental framework. Experiment 1: The study divided rats into control and MCAO/R groups to test memory impairment after modeling. The control group received no intervention, while the MCAO/R group was modeled using the suture method and tested with water maze or object recognition experiments 28 days later. Experiment 2: The study tested the continuous therapeutic effect by dividing MCAO/R rats into different groups and using normal rats as a control. After treatment, a water maze experiment was conducted for 6 days, followed by detection of IF, IHC, and TEM. Experiment 3: MCAO/R rats were divided into different groups for ELISA testing to verify the permeability of mNGF in various brain regions by SMES. Another set of MCAO/R rats were divided into groups for WB and ICH verification of the p65-VEGFA-TJs signal axis to explore the effect of SMES on opening the BBB of the hippocampus

Article Snippet: Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse Beta-NGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Permeability

Journal: Molecular Neurobiology

Article Title: Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood–Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats

doi: 10.1007/s12035-024-04337-8

Figure Lengend Snippet: The protein of mNGF expression in the M1 and various cognitive-related brain regions as measured by ELISA. A , B , C , D , E , F The NGF + SMES group had significant effect except PrL and MS. The results are demonstrated as the mean ± SEM ( n = 5, each group). * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. NGF + SMES group. G The hippocampus had significant effect. The results are demonstrated as the mean ± SEM ( n = 5, each group). ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. hippocampus group. a , b , c , d , e , f Compared with the MCAO/R group, NGF group, or NGF + SMES + PDTC group, NGF + SMES group had significant permeation effect in the M1, brain stem, and hippocampus. The results are demonstrated as the mean ± SEM ( n = 5 or 8, each group). * P < 0.05, ** P < 0.01, and **** P < 0.0001 vs. NGF + SMES group. g The hippocampus had significant effect. The results are demonstrated as the mean ± SEM ( n = 6, each group). **** P < 0.0001 vs. hippocampus group

Article Snippet: Following the manufacturer’s protocol, the NGF concentration in each brain sample was measured using a Mouse Beta-NGF Enzyme-linked Immunosorbent Assay (ELISA) Kit (NBP2-81189, Novus).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: eLife

Article Title: Senescence of endplate osteoclasts induces sensory innervation and spinal pain

doi: 10.7554/eLife.92889

Figure Lengend Snippet: ( a ) Enzyme-linked immunosorbent assay (ELISA) analysis showing the concentration of Netrin-1 in L3–5 endplates of adult sham, lumbar spine instability (LSI) + PBS, and LSI + ABT263 mice. ( b ) ELISA analysis showing the concentration of NGF in L3–5 endplates of adult sham, LSI + PBS, and LSI + ABT263 mice. n=3 per group. Statistical significance was determined by one-way ANOVA, and all data are shown as means ± standard deviations.

Article Snippet: We determined the concentration of Netrin-1 (LSBio, LS-F5882) and NGF (Boster, EK0470) in the L3–L5 endplates using the ELISA Development Kit according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay